I am a biochemistry professional specializing in molecular biology, cell culture, cancer research and development. A highly motivated and detail-oriented individual with a strong passion for science, seeking to begin a career as a Lab Technician.
Overview
6
6
years of professional experience
Work History
Lab Assistant
Hill Laboratories
, New Zealand
02.2025 - 06.2025
Accurately received, logged, processed, and tracked incoming samples according to strict laboratory protocols and time-sensitive requirements.
Maintained precise digital records and performed accurate data entry using specialized systems, ensuring data integrity.
Collaborated effectively with team members across different sections to ensure smooth operational workflow and timely sample progression.
Ensured equipment and work areas were maintained to high standards of cleanliness and operational readiness.
Research Technician/Assistant
Farcast Biosciences India Pvt Ltd
, India
07.2023 - 06.2024
Ex vivo culturing of cancer patient tissue for drug testing -Targeted therapy, Immunotherapy & Chemotherapy
Cryopreservation and revival of various tumor tissues and lead a project within a time frame.
Culturing of PBMC and checking functionality of immune cells using AntiCD3+IL2 and LPS stimulation
Isolation of PBMC and profiling of various immune cells using flowcytometry
Flowcytometry – Profiling of immune cells using 14 color panel
RNA & DNA isolation and PCR
Analysis using Graphpad Prism and Flowjo
Internal data presentation and review meeting.
Mammalian Cell culture – “BHK 21” and “HeLa” Cell line, cell migration assay, karyotyping, giemsa staining, functional design of Bioreactor and fermentor, cell revival, growth curve analysis, and media formulation.
Junior Research Fellow/Assistant
PSG Institute of Advanced Studies
, India
09.2019 - 07.2022
Project Title: "Microfluidic device-based instrument for screening breast cancer using miRNA detection from the serum"
RT-qPCR: Profiling miRNA isolated from serum in Breast cancer patients using PCR
Product Development: Microfluidics-based device manufactured by Molbio in collaboration with an industrial partner (Bigtec Labs, Bangalore).
MiRNA expression: Recorded the fluorescence value using a laser scriber-based device for miRNA-21 and miRNA-195 as a combination biomarker.
The molecular beacon probe hybridization technique was performed using a point-of-care device.
Gathered data from a patient who has strong pathological reports as well as cancer-affected individuals with proper counseling.
Expertise with scientific tools and instruments for data collection and experiments.
Education
Master of Science - Biochemistry
PSG College of Arts and Science
India
01.2019
Bachelor of Science - Biochemistry
KSR college of Arts and science
India
01.2017
Skills
Cell Culture: Ex vivo culture (3D model), tissue explant generation, PBMC isolation, Good Laboratory Practices (GLP)
Genomic and phenotypic Analysis : 14 color Flow cytometry, Fluorescent microscopy imaging
Biochemical and Analytical Techniques: Phytochemical analysis, HPLC, chromatography, enzyme assays protein purification, and differential centrifugation
Microbiology and cell culture: microbial plating, bacterial staining, antibiotic sensitivity assays, autoclaving and different inoculation methods
Software Tools: Data management and analysis include Excel, GraphPad Prism, mini tab, origin, SPSS, FlowJo, basics of Anaconda and Qpath for quantitative cell analysis (code creation and image analysis/Visualization)
Bioinformatics (Aspirational): Eager to develop proficiency in tools and languages for genomic and population genetic data analysis
References
Sabine, Shengleton, Supervisor, Sabine.hank@hotmail.com, 64274062128, Hill Labs, NZ
Kowshik, Jaganathan, Senior Scientist, Cancer Biology, kowshik.jaganathan@farcasbio.com, Farcast Biosciences India Pvt Ltd
Publications
Research Article: Sakthivel, K. M., Kokilavani, K., Kathirvelan, C., & Brindha, D. (2020). “Malvidin abrogates oxidative stress and inflammatory mediators to inhibit solid and ascitic tumor development in mice” - Journal of Environmental Pathology, Toxicology and Oncology, 39(3).
Review Article: Kokilavani, K., & Salim, B. Primary Diagnostic Tools for SARS-CoV-2 Infection (COVID-19): Mini Review Based on Current Challenges in Molecular Techniques and Point of Care Testing for Screening. International Journal of Pathogen Research (2020).
Research Article: B. Salim, M. Vijayakumar, K. Kokilavani, A. T. C. John and C. D. K, “Breast Cancer Screening by Blood Test using miRNAs as Biomarkers- a Point of Care Engineering Solution, Breast cancer screening by Blood Test using miRNAs as Biomarker- a point-of-care engineering solution. 2023”. International Conference for Advancement in Technology (ICONAT), Goa, India, 2023, pp. 1-5.
Research article: Krishnamoorthy Kokilavani1, Ancy Terryna Chanayiltharayil John1, Bindu Salim1, Madhulika Vijayakumar2, Sankar Ganesh Jeyaraj3,4: “Improved specificity for breast cancer screening using an oncogenic (miRNA-21) and a gene suppressor (miRNA-195) miRNA in the serum for a Point of Care (POC) screening solution”. Biomedical and Environmental Sciences 2023, 36(6) 549-55