Mayumi Harada (Yoshida)

Dynamic Research Technician with over 25 years of extensive experience in laboratory environments, excelling in various molecular biology research settings. Expertise encompasses advanced laboratory techniques, including ELISA and Western Blotting, combined with proficiency in cell culture and qPCR methodologies. Demonstrated ability to thrive under pressure while contributing to innovative research initiatives and maintaining high-quality standards. Committed to leveraging a strong background in veterinary medicine to support impactful scientific discoveries and advancements.

• Cancer research, Establishment of various tumour experimental models in vivo, Culture of cancer cell lines in vitro, Cell Proliferation and cell cytotoxicity measurement by MTT Assay
• Arthritis research, Measurement of arthritis in joints of experimental rats
• Wound Healing, Measurement of wound sizes in experimental rats, Histology of healed wounds
• Safety and Toxicity Studies, Monitoring of animals in chronic toxicity studies, Monitoring of animals and offspring in reproductive toxicity studies
• Apoptosis related research, DNA Fragmentation Assay, Flow Cytometry for Cell Cycle Analysis, Flow Cytometry for detection of apoptotic cells
• Angiogenesis related research, CAM (Chick Chorioallantoic Membrane) Assay, Mesenteric Windows Assay, Rat Aortic Rings Assay
• Immunological and Inflammatory Studies, Assay of interaction of SARS-CoV-2 spike protein with AEC2 receptor, Assay of Neutrophil activities, Measurement of Neutrophil NO production, Measurement of Neutrophil superoxide production, Measurement of Neutrophil TNF-α production, Measurement of Neutrophil Interleukins production, Assay of Monocyte and Macrophages activities, Isolation of murine resident peritoneal Macrophages, Isolation of rat blood Monocytes, Measurement of Macrophage NO production, Measurement of Macrophage Cytokine production by RT-PCR, Measurement of Macrophage Cytokine production by ELISA, Bioassay of TNF-α, Assay of Spleen Cells activities, Isolation of murine Spleen Cells and Incubation, Measurement of spleen cells cytokine productions by ELISA, Measurement of spleen cells cytokine production by RT-PCR, Immunohistological staining for tissue section samples
• Enzymological Studies, Assay for enzyme activities including Protease, tyrosinase, β-glucosidase, etc.
• Antioxidant studies, Assay for antioxidant, Measurement of lipid peroxide, Measurement of peroxide Value
• Protein related research, Assay for protein, Purification of proteins, SDS-PAGE, Western Blotting
• Diabetes related research, Assay of insulin released by ILETS
• Dermatological research, Skin absorption and transport studies, Skin reaction studies
• Cell culture, Culturing and monitoring of cell proliferation (fibroblasts, keratinocytes, epithelial cells)
• Bacteria culture, Culturing and monitoring of bacteria proliferation, Determination of the minimum inhibitory concentration on micro-organisms

• Regional distribution and plasma concentration of peptide YY in sheep, Yoshida M, Onaga T, Inoue H, Yokota H, Kato S, Rakuno Gakuen University, Japan, 11/21/98
• The changes in plasma concentration of peptide YY and the regional distribution in sheep, Onaga T, Yoshida M, Inoue H, Yokota H, The 128th Annual Meeting of Japan Association for Veterinary Medicine, Kumamoto, Japan, 10/13-15/99
• GCP has potent anti-angiogenic activity in addition to its anti cancer activity, Davis PF, Sun B, Gush J, Yoshida M, Yuan L, The 9th Symposium of AHCC Research Association, Sapporo, Japan, 08/19-20/01
• A Novel Mechanism of GCP: Involvement of b-glucuronidase (heparanase), Yuan L, Miura T, Yoshida M, Wagatsuma C, The 9th Symposium of AHCC Research Association, Sapporo, Japan, 08/19-20/01
• GCP; A soybean Isoflavone dietary supplement fermented by Basidiomycetes with anticarcinogenic activities: their mechanism and chemopreventive potential, Yuan L, Miura T, Sun B, Yoshida M, Wagatsuma C, Fuji H, Kosuna K, International Symposium on Dietary and Medicinal Antimutagens and Anticarcinogens: Molecular Mechanisms and Chemopreventive Potential, Seoul, Korea, 10/17-19/01
• Anti-tumor and anti-angiogenic effects of GCP (Genistein Concentrated Polysaccharide), Wagatsuma C, Yuan L, Sun B, Yoshida M, Fuji H, The 13th Annual Meeting of The Japan Society for Biological Therapy, Kyoto, Japan, 12/06-07/01

• GCP: Genistein concentrated polysaccharide with anti-angiogenesis and anti-tumor activities in vitro and in vivo, Yoshida M, Yuan L, Miura T, Kosuna K, The 10th Alternative therapy conference, Tokyo, Japan, 10/10/00
• GCP: A genistein concentrated polysaccharide with antiangiogenesis and antitumor activities in vitro and vivo, Yuan L, Miura M, Sun B, Yoshida M, Fuji H, Kosuna K, Davis PF, Hamada J, The 92nd Annual Meeting of American Association for Cancer Research, New Orleans, USA, 03/01
• The mechanism of GCP (Genistein Concentrated Polysaccharide) against mice with tumor: Involvement of beta-glucuronidase (heparanase), Yuan L, Wagatsuma C, Sun B, Yoshida M, Fuji H, The 60th Annual Meeting of Japan Society of Cancer Research, Yokohama, Japan, 09/26-28/01

• Regional distribution and plasma concentration of peptide YY in sheep, Onaga T, Yoshida M, Inoue H, Yokota H, Peptides, 05/2000, 21, 5, 655-67
• Mechanism of Anti-Angiogenic Activity of a Potent Lipid-Rich Extract from Sharks, Lan Yuan, Mayumi Yoshida and Paul F Davis, Journal of Medicinal Food, 2006, 9, 3, 300-6

• Self-growth and leading others
• Walking
• Gardening
• Skiing
• Cooking
• Badminton
• Running
• Mountain biking
• Dog breeding

Research Technician, Trinity Bioactives Ltd, 69 Gracefield Road, Gracefield, Lower Hutt, New Zealand

National licenced Veterinarian in Japan