Targeted Cancer Therapeutics Development
University of Auckland Laboratory Project
Project Title: Selective Binding of Therapeutic Antibodies to Mutant c-MET in Human Cells
Key Techniques: Immunocytochemistry, ELISA, Flow Cytometry, Sequence Alignment, Fluorescence Microscopy
- Investigated the binding affinity of newly developed therapeutic antibodies to wild-type and mutant c-MET, a tyrosine kinase linked to cancer progression.
- Performed comparative expression and localisation analyses in human DNA biopsy samples using fluorescence imaging and GFP-tagged constructs.
- Applied ELISA and flow cytometry to quantitatively assess antibody binding strength; identified antibody B as most selective for mutant c-MET.
- Conducted sequence alignment to locate a deletion mutation in the intracellular juxtamembrane region of mutant c-MET, associated with altered protein function.
- Concluded that mutant c-MET exhibits more diffuse localisation and higher fluorescence intensity, potentially influencing therapeutic targeting efficacy.
_____________________________________________________________________________
Influenza Virus Characterisation Using HA and HAI Assays
University of Auckland Laboratory Project
Project Title: Quantitation and Inhibition Analysis of Human Influenza Virus Strains
Key Techniques: Hemagglutination (HA) Assay, Hemagglutination Inhibition (HAI) Assay, Serial Dilutions, Virus Quantification
- Conducted HA assays to determine viral concentration endpoints for Influenza B (IBV), H1N1, and H3N2 strains, measuring hemagglutination titers of up to 640 HA units/mL.
- Performed HAI assays to assess antibody-mediated inhibition of viral agglutination, accurately identifying one HAI unit per antiserum and quantifying antibody efficacy at various dilutions.
- Analysed effects of temperature and viral concentration on assay sensitivity and inhibition patterns, interpreting shifts in hemagglutination based on HA/NA protein function.
- Investigated antigenic drift and shift by exploring genetic changes in viral surface proteins (HA and NA), linking point mutations and genome reassortment to the emergence of new strains.
- Gained practical skills in serological virus typing, antigenic variation analysis, and interpretation of immune response assays under controlled experimental conditions.
_____________________________________________________________________________
Diagnostic Identification of Staphylococcus aureus
University Laboratory Project
Project Title: Identification of Staphylococcus aureus in a 52-Year-Old Patient
Key Techniques: Gram Staining, Catalase Test, Coagulase Test, Mannitol Salt Agar Culture, Clinical Correlation
- Investigated a clinical case involving necrotising skin lesions and systemic infection symptoms (e.g., metabolic acidosis, high CRP, DIC) in a post-biopsy patient.
- Performed diagnostic testing to identify an unknown bacterial isolate from a skin biopsy, progressing through a structured workflow to exclude fungal and Gram-negative organisms.
- Conducted a Gram stain confirming Gram-positive cocci, followed by a catalase test (positive), narrowing identification to Staphylococcus species.
- Used coagulase testing to distinguish S. aureus from coagulase-negative staphylococci; observed clot formation confirming S. aureus.
- Validated results using Mannitol Salt Agar (MSA); yellow color change confirmed mannitol fermentation consistent with S. aureus.
- Linked clinical presentation (hemorrhagic bullae, necrosis) to the pathogen's ability to produce virulence factors such as hyaluronidase and coagulase, enabling immune evasion and tissue destruction.